Figure 2
From: Identification and characterization of naturally occurring splice variants of SAMHD1
Immunofluorescence analysis of SAMHD1 WT and splice variants. HeLa cells were either mock transfected (panel A) or transfected with 1 μg of pcDNA-SAMHD1 WT (panel B), 5 μg of Δ14 (panel C), or 4 μg of Δ8-9 (panel D). Total amounts of transfected DNA were adjusted to 5 μg with empty vector DNA as necessary. After the transfection, cells were replated onto cover slips, grown for 24 h, and then fixed with methanol (10 min, -20°C). Cells were dually stained with rabbit polyclonal antibody to SAMHD1 (SAM416; red) and a mouse monoclonal antibody to lamin-B (green). Samples were analyzed on a confocal microscope as detailed in Methods.