Figure 1
HIV-1 impairs apoA-I mediated cholesterol efflux from THP-1 cell-differentiated macrophages. (A) HIV AD8 infected and uninfected THP-1 cell-differentiated macrophages were cultured for 14 days. MOI represents multiplicity of infection. Cells were labeled with [3H] cholesterol, and subsequently incubated with media in the presence and absence of apoA-I (50 μg/ml). ApoA-I-induced cholesterol efflux was measured and analyzed as described in the Materials and Methods. (B) Cells were treated the same as above but incubated with medium in the presence or in absence of HDL (50 μg/ml) and HDL mediated cholesterol efflux was measured. (C) ApoA-I mediated cholesterol efflux was determined in the presence and absence of the HIV replication inhibitor AZT (5uM). All experiments were performed in triplicate, and results shown are mean ± SD with P values. (D) Uninfected or infected with HIV AD8 (moi 0.01) and HIV Bal (moi 0.001) THP-1 cell-differentiated macrophages cultured for 10 days then incubated with AcLDL (50μg/ml) for 48 h followed by 30μg/ml apoA-I stimulation for 18 hours. Lipid accumulation was determined by Oil red O staining by light microscopy. HIV reverse transcriptase activity in culture medium is shown in the bottom panel.