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Figure 6 | Retrovirology

Figure 6

From: The Gammaretroviral p12 protein has multiple domains that function during the early stages of replication

Figure 6

Infectivity of Mo-MLV and GaLV with single amino acid changes in p12. (A) An alignment of the residues comprising the N- and C-terminal domains of p12 in Mo-MLV and GaLV is shown. Colour shading indicates the original Mo-MLV p12 substitution mutants synthesized. (B and C) Single alanine substitutions were made for each residue within these domains for Mo-MLV p12 (B) or GaLV p12 (C). LacZ-encoding mutant VLPs were synthesized and equivalent RT-units of VLPs were used to challenge D17 cells. Infectivity was measured by detection of β–galactosidase activity in a chemiluminescent reporter assay. Results are plotted as the percentage of infectivity compared to the infectivity of the corresponding wild type VLPs for each species. The mean and range of three independent experiments are shown.

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