Figure 5

The effect of inserting a chromatin binding domain into p12. Sequence coding for the 14 amino acid PFV chromatin binding sequence (CBS) was inserted into the middle of p12 in Mo-MLV Gag-Pol expression vectors carrying wild type p12 or p12 mutants 6, 7, 14 or 15 following introduction of an AscI site. These plasmids were used to synthesize LacZ-encoding VLPs in 293T cells. D17 cells were challenged with equal RT-units of VLPs and infectivity was measured by detection of β–galactosidase activity in a chemiluminescent reporter assay. Infectivity is plotted as a percentage of the infectivity of Mo-MLV containing wild type p12 without the introduced AscI site. The mean and range of three independent experiments are shown.