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Figure 4 | Retrovirology

Figure 4

From: Translation of MMTV Gag requires nuclear events involving splicing motifs in addition to the viral Rem protein and RmRE

Figure 4

Negative regulation against Mtv-1 and HBRV gag acts at the transcript level. A) The gag ORF was cloned in the multiple cloning site of pIRES-eGFP upstream of the IRES and GFP, and was under the control of the CMV promoter. B) Expression of GFP was visualized by fluorescence microscopy. The "empty" pIRES-eGFP construct was used as a control for GFP expression. C) Gag levels were quantified by pulse labeling and immunoprecipitation with an MMTV anti-CA antibody. Pr77gag, the Gag precursor (77 KDa).

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