Figure 5

Cross-neutralizing Breadth and Potency against HIV-1 Subtype C Env Reference Panel. Bolded individuals represent superinfected individuals with evidence of superinfection from outsider partnerships (ZM282M, ZM211F and ZM247F) or non-superinfected controls who self-reported outside partnerships. Pre-superinfection plasma for superinfected individuals or similar plasma time points for non-superinfected controls was tested against a panel of twelve Subtype C envelope pseudoviruses. This panel included Envs of both Tier 1b and Tier 2 sensitivities. Starting plasma dilution was reduced to 1:20 to increase assay sensitivity. Plasma-env combinations, which did not reach an IC50 value at the lowest dilution tested (1:20), were assigned a value of 10. Breadth score was calculated by adding the total number of envelopes neutralized at an IC50 greater than or equal to 20. Potency score was calculated by dividing individual plasma-env IC50 by median IC50 per envelope against all plasma and then adding the sum of these scores (rounded to the nearest integer) for each plasma. “Auto” indicates that a plasma sample was tested against an autologous envelope in the panel, autologous IC50 values were not counted in breadth and potency scores.