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Figure 9 | Retrovirology

Figure 9

From: Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles

Figure 9

Loss of Gag-Env colocalization in FVs over time in live cells. HeLa cells were incubated with double-tagged viruses composed of Gag:Gag-eGFP labeled capsids (3:1) and PE Ch (PE Ch, blue), PE Ch iCS (PE Ch iCS, black) or SE Ch (SE Ch, magenta) Env proteins and imaged by 3D spinning-disk confocal microscopy at different time points after warming the cells to 37°C. Z-stacks with 30 planes were collected every 5 to 10 min. The data points at 0 min represent the colocalization percentage obtained from the corresponding sample preparations directly spotted on a cover slide (as shown, for example, in Figure 1D). The colocalization percentage obtained from several HeLa cells for each of the three virus strains is shown as a function of time. The colocalization percentage was determined by grouping the measurements into subsets of 2–4 cells and calculating the average percentage (circle) of colocalization and the standard deviation of the mean (error bars) (PE Ch: N = 13, 5 subsets; PE Ch iCS: N = 16, 5 subsets; SE Ch: N2009= 23, 7 subsets). The results were averaged into 10-minute time bins. Lines are drawn between data points as a guide to the eye

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