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Figure 5 | Retrovirology

Figure 5

From: Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles

Figure 5

Time-lapsed imaging of PFV uptake. HeLa cells were infected with double-tagged viruses consisting of PFV Gag-eGFP and (A, B) PE Ch or (C) PE Ch iCS. Panel A and C display images from differential interference contrast measurements (DIC) as well as an overlay of the eGFP and mCherry channels (merge) for the selected time points of 0 h, 1 h, 4 h, 12 h and 24 h post virus binding. The fluorescence images are maximum intensity z-projections of z-stacks of 10–12 planes collected using live-cell wide-field microscopy. The scale bar represents 10 μm. Panel B shows an expanded region along with orthogonal views of the perinuclear area from images in panel A at 1 h, 4h and 24 h post virus binding. maxP, maximum intensity projection. See Additional file 3 and Additional file 5

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Retrovirology

ISSN: 1742-4690

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