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Figure 2 | Retrovirology

Figure 2

From: Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles

Figure 2

Schematic outline of FV mCherry-Env fusion proteins and Env cleavage site mutants. The mCherry tag (Ch) with a flexible glycine-serine-linker (L) was fused to the N-terminus of the env open reading frames of (A) PFV or (B) SFVmac. (C) Inactivation of the surface-transmembrane cleavage site (SU/TM) was achieved by introducing a single point mutation (R571T) in the PE/PE Ch sequence or exchanging four amino acids (RKRR570-573AAEA) in the SE/SE Ch sequence. These mutations resulted in PE iCS and SE iCS or the mCherry-Env fusion constructs of PE Ch iCS and SE Ch iCS. The cleavage sites in the Env proteins are indicated as dashed lines. Abbreviations: LP, leader peptide; PE, PFV Env; SE, SFVmac Env; Ch, mCherry; iCS, inactivated cleavage site

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