Figure 6

Immune response in immunized mice. (a) HIV-1-_JR-FL envelope T-cell responses measured by the IFN-γ ELISPOT assay on peripheral blood cells from mice immunized with IDLV-JR (black bars), IDLV-JR/Vpx (grey bars) and naïve mice (empty bars) at 6 and 9 weeks from injection. Analysis was performed on blood cells stimulated overnight with H2-D^d restricted specific Envelope JR-9mer peptide, as described in the Methods section. IFN-γ producing T cells are expressed as SFC/10⁶ cells after background subtraction. The error bars indicate the standard deviation among mice of the same group, and P-values are indicated for intergroup comparisons. (b) Anti-gp120 and anti-p24 IgG measured by ELISA in plasma samples of mice immunized with IDLV-JR (black bars) or IDLV-JR/Vpx (grey bars) at 6 weeks after immunization. Plasma samples from immunized mice were analyzed separately. Results are expressed as the IgG mean titer of 4 mice per group. Error bars indicate the standard deviations amongst mice of the same group. P-values are indicated for intergroup comparisons.