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Figure 4 | Retrovirology

Figure 4

From: Clinical, virological and biochemical evidence supporting the association of HIV-1 reverse transcriptase polymorphism R284K and thymidine analogue resistance mutations M41L, L210W and T215Y in patients failing tenofovir/emtricitabine therapy

Figure 4

Rescue DNA polymerization initiated from AZTMP-, d4TMP-, and tenofovir-terminated primers annealed to an RNA template. Time course experiments of excision reactions were carried out in the presence of 3.2 mM ATP. The nucleotide sequences of template-primers used are given above their corresponding graphs. All dNTPs in the assays were supplied at 200 μM, except for dATP or dTTP (depending on the reaction) whose concentration was 2 μM. Template-primer and active RT concentrations in these assays were 30 and 24 nM, respectively. The values (averages standard deviations [error bars]) were obtained from three independent experiments.

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