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Figure 3 | Retrovirology

Figure 3

From: Single mutations in the transmembrane envelope protein abrogate the immunosuppressive property of HIV-1

Figure 3

Cytokine release from PBMCs of healthy donors in response to exposure to the wt gp41 (black) and the mutated gp41 (gray) as estimated by ELISA. (A) Release of IL-10 from PBMCs of 4 donors measured in triplicates. Wt - wild-type gp41, mock - transfection with the control vector pcDNA3(−), n.d. - not done. PBMCs from donor 1 were tested twice within a three month interval. (B) Comparison of medians of IL-10 release from all donor PBMCs and determination of the P values for the influence of each mutant on IL-10 release in comparison with the wt gp41. Error bars represent standard deviations of sample medians, for the P values the Student’s t test was used. 6A* - More data are required to make a statistical analysis. (C) Schematic presentation of the influence of mutations in the isu domain on IL-10 release, large letters indicate abrogation of IL-10 release (core region), small letters - less inhibition, amino acids given in gray (RYLK) were not investigated. Numbers underlined indicate amino acid residues crucial for both, IL-10 and IL-6 release. (D) Release of IL-6 from PBMCs of donor 4 after exposure to wt gp41 and mutated gp41s as estimated by ELISA.

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