Figure 4

PRMT5 knockdown and HTLV gene expression. A) 293T cells expressing normal and knockdown levels of PRMT5 were transfected with HTLV-1 molecular clone. Immunoblotting was performed using anti-PRMT5 antibodies to confirm the knockdown and with anti-HTLV p24 antibodies to monitor p24 levels. The amount of p19 Gag production was analyzed by p19 ELISA. B) 293T cells transduced with scrambled shRNA or shRNA against PRMT5 were transfected with HTLV-2 molecular clone. Knockdown of PRMT5 was confirmed by immunoblotting with anti-PRMT5 antibodies. The intracellular p24 and extracellular p19 Gag production was analyzed by immunoblotting with anti-HTLV p24 antibodies and with p19 ELISA respectively. Student t-test resulted in a p value of 0.011 (_*) indicating that decrease in p19 production with lower levels of PRMT5 is significant. Immunoblotting with anti-Actin antibodies was used to validate equal loading.