Skip to main content
Figure 3 | Retrovirology

Figure 3

From: Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses

Figure 3

Validation of p30 and p28 interaction with PRMT5. Mock, S-GFP, S-p30-HA and S-p28-HA expressed in 293T cells were purified by S-tag affinity purification and immunoblotting with anti-PRMT5 antibody. The expression of PRMT5 was confirmed by immunoblotting the cell lysates with anti-PRMT5 antibody. B) 293T cells co- transfected with p30 or p28 or Flag PRMT5 as indicated in the figure. Immunoprecipitation was performed using anti-Flag antibodies and subsequently probed for p30 and p28 using anti-HA antibodies. The expression of Flag-PRMT5, p30 and p28 was validated by immunoblotting as shown in the figure.

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us