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Figure 2 | Retrovirology

Figure 2

From: Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses

Figure 2

Validation of p28 interaction with hnRNP H1. A) Ectopically expressed Mock, S-GFP, S-p30-HA, S-p28-HA in 293T cells were S-tag affinity purified and probed with anti-hnRNP H1 antibodies. Endogenous expression of hnRNP H1 was confirmed by immunoblotting with anti-hnRNP H1 antibody. B) The immunoprecipitation assay was performed on cell lysates transfected with S-p30-HA and S-p28-HA. Non-specific IgG (NSIgG) or anti-hnRNP H1 antibodies were used for immunoprecipitation and subsequently probed with anti-HA antibody. Immunoblotting using anti-HA and anti-hnRNP H1 was used to validate the expression of all proteins.

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