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Figure 1 | Retrovirology

Figure 1

From: Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses

Figure 1

Validation of p30 interaction with REGγ and NIP30. A) S-tag affinity purification with Mock, S-GFP, S-p30-HA and S-p28-HA transfected 293T cell lysates. The purified product was analyzed by immunoblotting using indicated antibodies. The expression of individual protein and S-tag purification was confirmed using the indicated antibodies. B) 293T cells were transfected with S-p30-HA and S-p28-HA and immunoprecipitated with non-specific IgG (NSIgG) or anti-REGγ antibody and probed with anti-HA antibody. Immunoblotting of input using anti-REGγ and anti-HA antibodies confirmed the expression of the interacting proteins. C) 293T cells lysates expressing S-p30-HA and S-p28-HA were immunoprecipitated with non-specific IgG (NSIgG) or anti-NIP30 antibody and probed with anti-HA antibody. The expression of p30, p28 and NIP30 was confirmed with indicated antibodies.

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Retrovirology

ISSN: 1742-4690

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