|
Virus
|
Recipient cells
|
Trial 1
|
Trial 2
|
Trial 3
|
Trial 4
|
Ave ± Std
|
|---|
|
IU/ml*
|
IU/ml
|
IU/ml
|
IU/ml
|
IU/ml
|
Relative infectivity**
|
|---|
|
XMRV
|
293/pcDNA3
|
2.5 X 106
|
2.9 X 106
|
1.7 X 106
|
2.9 X 106
|
2.5 ± 0.52 X 106
|
1 ± 0
|
| |
293/hA3B
|
2.2 X 106
|
2.3 X 106
|
2.5 X 106
|
4.3 X 106
|
2.8 ± 0.97 X 106
|
1.17 ± 0.365
|
|
MX
|
293/pcDNA3
|
6.5 X 106
|
6.9 X 106
|
5.1 X 106
|
8.3 X 106
|
6.7 ± 1.3 X 106
|
2.73 ± 0.231
|
| |
293/hA3B
|
6.9 X 106
|
9.0 X 106
|
3.6 X 106
|
8.2 X 106
|
6.9 ± 2.4 X 106
|
2.72 ± 0.462
|
- The 293 cells transfected with the plasmid encoding HA-tagged hA3B plasmids (293/hA3B) or control plasmids (293/pcDNA3) were selected by G418 and were used as recipient cells for infectivity assay. The recipient cells were infected with XMRV or MX viruses that were produced from the infected 293 T cells. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the 293/pcDNA3 infected with XMRV viruses was shown.
