Moloney murine leukemia virus glyco-gag facilitates xenotropic murine leukemia virus-related virus replication through human APOBEC3-independent mechanisms

Table 4 Expression of hA3B in 293 cells did not alter infectivity of XMRV or MX viruses

Virus	Recipient cells	Trial 1	Trial 2	Trial 3	Trial 4	Ave ± Std
Virus	Recipient cells	IU/ml^*	IU/ml	IU/ml	IU/ml	IU/ml	Relative infectivity^**
XMRV	293/pcDNA3	2.5 X 10⁶	2.9 X 10⁶	1.7 X 10⁶	2.9 X 10⁶	2.5 ± 0.52 X 10⁶	1 ± 0
	293/hA3B	2.2 X 10⁶	2.3 X 10⁶	2.5 X 10⁶	4.3 X 10⁶	2.8 ± 0.97 X 10⁶	1.17 ± 0.365
MX	293/pcDNA3	6.5 X 10⁶	6.9 X 10⁶	5.1 X 10⁶	8.3 X 10⁶	6.7 ± 1.3 X 10⁶	2.73 ± 0.231
	293/hA3B	6.9 X 10⁶	9.0 X 10⁶	3.6 X 10⁶	8.2 X 10⁶	6.9 ± 2.4 X 10⁶	2.72 ± 0.462

The 293 cells transfected with the plasmid encoding HA-tagged hA3B plasmids (293/hA3B) or control plasmids (293/pcDNA3) were selected by G418 and were used as recipient cells for infectivity assay. The recipient cells were infected with XMRV or MX viruses that were produced from the infected 293 T cells. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the 293/pcDNA3 infected with XMRV viruses was shown.

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