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Table 2 The mutations for putative glyco-gag did not influence anti-viral activity of hA3G in XMRV infection

From: Moloney murine leukemia virus glyco-gag facilitates xenotropic murine leukemia virus-related virus replication through human APOBEC3-independent mechanisms

Virus

hA3G

Trial 1

Trial 2

Ave ± Std

IU/ml*

Relative infectivity**

IU/ml

Relative infectivity

IU/ml

Relative infectivity

VP62

-

1.7 X 104

1

1.4 X 104

1

1.5 X 104 ± 2.3 X 103

0.209 ± 0.029

 

+

3.2 X 103

0.188

3.2 X 103

0.229

3.2 X 103 ± 3.3 X 101

 

VP62Δgg0

-

8.4 X 103

1

1.2 X 104

1

1.0 X 104 ± 2.3 X 103

0.196 ± 0.058

 

+

1.3 X 103

0.155

2.8 X 103

0.237

2.0 X 103 ± 1.1 X 103

 

VP62Δgg1

-

1.2 X 103

1

3.4 X 103

1

2.3 X 103 ± 1.5 X 103

0.240 ± 0.014

 

+

3.0 X 102

0.250

7.7 X 102

0.230

5.4 X 102 ± 3.3 X 102

 

VP62Δgg2

-

1.8 X 104

1

9.1 X 103

1

1.3 X 104 ± 6.0 X 103

0.234 ± 0.010

 

+

4.0 X 103

0.227

2.2 X 103

0.241

3.1 X 103 ± 1.3 X 103

 

VP62Δgg3

-

1.1 X 104

1

8.2 X 103

1

9.5 X 103 ± 1.8 X 103

0.214 ± 0.068

 

+

2.8 X 103

0.262

1.4 X 103

0.165

2.1 X 103 ± 1.0 X 103

 

VP62Δgg4

-

2.2 X 104

1

1.3 X 104

1

1.7 X 104 ± 6.2 X 103

0.258 ± 0.049

 

+

6.4 X 103

0.292

2.9 X 103

0.223

4.7 X 103 ± 2.5 X 103

 
  1. The 293 T cells were transfected with the XMRV expressing plasmid pVP62 along with the hA3G-V5 or control (pcDNA3) plasmids. The viruses were gathered 2 days post-transfection and were used for infectivity assay with HeLa cells. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the HeLa cells infected with the viruses released from the 293 T cells transfected with control plasmid was shown.