|
Virus
|
hA3G
|
Trial 1
|
Trial 2
|
Ave ± Std
|
|---|
|
IU/ml*
|
Relative infectivity**
|
IU/ml
|
Relative infectivity
|
IU/ml
|
Relative infectivity
|
|---|
|
VP62
|
-
|
1.7 X 104
|
1
|
1.4 X 104
|
1
|
1.5 X 104 ± 2.3 X 103
|
0.209 ± 0.029
|
| |
+
|
3.2 X 103
|
0.188
|
3.2 X 103
|
0.229
|
3.2 X 103 ± 3.3 X 101
| |
|
VP62Δgg0
|
-
|
8.4 X 103
|
1
|
1.2 X 104
|
1
|
1.0 X 104 ± 2.3 X 103
|
0.196 ± 0.058
|
| |
+
|
1.3 X 103
|
0.155
|
2.8 X 103
|
0.237
|
2.0 X 103 ± 1.1 X 103
| |
|
VP62Δgg1
|
-
|
1.2 X 103
|
1
|
3.4 X 103
|
1
|
2.3 X 103 ± 1.5 X 103
|
0.240 ± 0.014
|
| |
+
|
3.0 X 102
|
0.250
|
7.7 X 102
|
0.230
|
5.4 X 102 ± 3.3 X 102
| |
|
VP62Δgg2
|
-
|
1.8 X 104
|
1
|
9.1 X 103
|
1
|
1.3 X 104 ± 6.0 X 103
|
0.234 ± 0.010
|
| |
+
|
4.0 X 103
|
0.227
|
2.2 X 103
|
0.241
|
3.1 X 103 ± 1.3 X 103
| |
|
VP62Δgg3
|
-
|
1.1 X 104
|
1
|
8.2 X 103
|
1
|
9.5 X 103 ± 1.8 X 103
|
0.214 ± 0.068
|
| |
+
|
2.8 X 103
|
0.262
|
1.4 X 103
|
0.165
|
2.1 X 103 ± 1.0 X 103
| |
|
VP62Δgg4
|
-
|
2.2 X 104
|
1
|
1.3 X 104
|
1
|
1.7 X 104 ± 6.2 X 103
|
0.258 ± 0.049
|
| |
+
|
6.4 X 103
|
0.292
|
2.9 X 103
|
0.223
|
4.7 X 103 ± 2.5 X 103
| |
- The 293 T cells were transfected with the XMRV expressing plasmid pVP62 along with the hA3G-V5 or control (pcDNA3) plasmids. The viruses were gathered 2 days post-transfection and were used for infectivity assay with HeLa cells. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the HeLa cells infected with the viruses released from the 293 T cells transfected with control plasmid was shown.
