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Table 6 SIV-specific antibody responses in plasma at the end of tenofovir treatment and after tenofovir withdrawal

From: Prolonged tenofovir treatment of macaques infected with K65R reverse transcriptase mutants of SIV results in the development of antiviral immune responses that control virus replication after drug withdrawal

   SIV-specific binding IgG1 Neutralizing antibody ID50 in TZM-bl cells2 ADCVI3
% inhibition of 1:100 serum dilution
Animal number Time point   SVA-MLV SIVmac239CS.23 SIVmac251-CS SIVmac251-TCLA SIVmac251-CS SIVmac251-TCLA SIVmac251 (UCD) RT-SHIV
29276 Euthanasia 409,600 20 <10 17 29,462 48.89 99.98 99.99 nd
30577 TFV withdrawal 409,600 <10 <10 12 380,644 61.77 99.94 93.49 99.89
Euthanasia 409,600 <10 13 11 >1,562,500 33.29 99.94 94.48 99.89
32186 TFV withdrawal 1,638,400 <10 <10 10 >1,562,500 99.98 99.98 99.99 nd
Euthanasia 1,638,400 <10 <10 <10 >1,562,500 99.98 99.98 99.06 nd
33088 TFV withdrawal 409,600 <10 <10 13 451,911 99.98 99.98 99.99 nd
Euthanasia 409,600 <10 <10 <10 >1,562,500 99.78 99.98 99.99 nd
33091 TFV withdrawal 6,553,600 <10 <10 10 >1,562,500 99.98 99.98 99.99 nd
Euthanasia 6,553,600 <10 <10 12 >1,562,500 99.98 99.98 99.99 nd
  1. 1Values of SIV-binding antibody titers are the reciprocal of the highest plasma dilution (of 4-fold dilutions) that gave a positive signal on whole-virus antibody ELISA assay specific for IgG.
  2. 2Values of neutralizing antibody are the serum dilution at which relative luminescence units (RLUs) were reduced 50% compared to virus control wells (no test sample); viruses tested were pseudoviruses SIVmac239CS.23 and SVA-MLV grown in 293 T cells, and replication-competent SIVmac251-CS (hPBMC-grown) and uncloned SIVmac251-TCLA (H9-grown; tissue culture lab adapted).
  3. 3ADCVI: antibody-dependent cell-mediated virus inhibition was tested at a 1:100 serum dilution with SIV-infected CEM.NKr R5 as targets and human PBMC as effector cells. The reported values of % inhibition are the averages of 2 independent experiments. The SIVmac251CS and SIVmac251-TCLA are the same as those used in the neutralizing antibody assay; SIVmac251 (UCD) is a stock grown in rhesus PBMC derived from the same seed stock as the SIVmac251 stock that was used to inoculate animals 32186, 33088 and 33091. The RT-SHIV stock was grown in rhesus PBMC from the same seed stock used to infect animal 30577. Only serum of RT-SHIV infected animal 30577 was tested for ADCVI activity against RT-SHIV. nd indicates not done.