Heterologous protection elicited by candidate monomeric recombinant HIV-1 gp120 vaccine in the absence of cross neutralising antibodies in a macaque model

Table 5 Detection of virus following transfer of immune serum from W61D rgp120 immunised macaques and challenge with SHIV _sbg (50 MID50 dose)

GROUP	ID NUMBER	TREATMENT	OUTCOME OF CHALLENGE (Weeks Post Challenge)
			−2		2		4	9	10	20
			VI	VI	VT*	PCR	PCR	VI	VI	VI
E	C37	50 ml pooled serum from A71, A75, A76, A78 i.p day −1	-	+	2.5	-	+	+	ND	-
	C38		-	+	2.0	+	+	+	ND	+
	C39		-	+	2.0	+	+	+	ND	-
	C40		-	+	1.5	+	+	+	ND	-
F	C41	None	-	ND	2.5	+	+	ND	-	-
	C42		-	ND	1.0	-	+	ND	-	-
	C43		-	ND	3.0	+	+	ND	-	-
	C44		-	ND	3.0	+	+	ND	+	-

VT* = Virus Titration; Number of SIV + ve cells per 10⁶ PBMC.
VI = Virus isolation by co-culture of 5 x 10⁶ PBMC with 10⁵ C8166 indicator cells.
PCR = Diagnostic DNA PCR specific for SIV gag.
ND = Assay not performed.

ISSN: 1742-4690