Figure 5

MOV10 is not necessary for miRNA or siRNA-mediated mRNA silencing. (A) MOV10 is not necessary for miRNA-mediated mRNA repression. HeLa non-silencing control or MOV10 KD cells were co-transfected with either FF4LCS (let-7 WT) or FFr4mLCS (let-7 mutant) together with pRenilla. Relative luciferase activities were measured using a Dual-Luciferase® Reporter Assay System and FF luciferase activity was normalised to the renilla luciferase activity. (B) MOV10 is not required for siRNA-mediated mRNA cleavage. HeLa non-silencing control or MOV10 KD cells were transfected with either psi-CHECK2-let-7X3 (let-7 WT) or psi-CHECK2-let-7X3m (let-7 mutant). Luciferase activities were measured as described in panel (A) and renilla luciferase activity was normalised to FF luciferase activity. Results are normalised to the non-silencing control, which is set at 100%. Values are the mean ± SD of 3 independent experiments.