Figure 2
From: A mutant retroviral receptor restricts virus superinfection interference and productive infection
The binding affinity of GALV RBD with MDTF cells expressing HA tagged PiT1, PiT2K522E or PiT2. GALV RBD tagged with both V5 and His was purified using high-performance nickel-NTA (Ni-NTA) agarose, serially diluted four times and applied to a binding assay for MDTFPiT1-HA (A) or MDTFPiT2K522E-HA and MDTFPiT2-HA cells (B). The kinetics of binding of GALV RBD was compared. The mean fluorescence intensity (MFI) for GALV RBD binding is indicated on the y-axis and GALVRBD concentration (mg) on the x-axis. Three independent experiments were performed and the mean ± standard deviation was presented in the curve.