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Table 1 Immunostaining data

From: Role of SAMHD1 nuclear localization in restriction of HIV-1 and SIVmac

SAMHD1 Experiment 1 Experiment 2 Experiment 3 Figure
  Exclusively nuclear Exclusively cytoplasm Throughout cell Exclusively nuclear Exclusively cytoplasm Throughout cell Exclusively nuclear Exclusively cytoplasm Throughout cell  
GFP-SAMHD1 184 0 16 190 0 10 188 0 12 1B
GFP-SAMHD1 (1–328) 176 0 24 180 0 20 177 0 23 1B
GFP-SAMHD1(329-626 0 40 160 0 46 154 0 38 162 1B
GFP-SAMHD1(1–150) 156 0 44 160 0 40 164 0 36 1B
GFP-SAMHD1(151–328) 0 0 200 0 0 200 0 0 200 1B
GFP-SAMHD1 K11A 0 188 12 0 190 10 0 192 8 2B
GFP-SAMHD1 R12A 0 190 10 0 194 6 0 188 12 2B
GFP-SAMHD1 R14A 0 192 8 0 196 4 0 186 14 2B
SAMHD1-FLAG 196 0 4 194 0 6 196 0 4 2D
SAMHD1-FLAG K11A 0 194 6 0 192 8 196 0 4 2D
SAMHD1-FLAG R12A 0 196 4 0 194 6 0 192 4 2D
SAMHD1-FLAG R14A 0 192 8 0 190 10 0 188 12 2D
GFP-muNS 0 200 0 0 200 0 0 200 0 3A
KRPR-GFP-muNS 150 0 50 146 0 54 152 0 48 3A
GFP 0 0 200 0 0 200 0 0 200 3B
KRPR-GFP 140 0 60 146 0 54 142 0 58 3B
SAMHD1-FLAG (U937) 194 0 6 190 0 10 196 0 4 4C
SAMHD1-FLAG K11A (U937) 194 0 6 190 0 10 196 0 4 4C
SAMHD1-FLAG R12A (U937) 0 192 8 0 196 4 0 188 14 4C
SAMHD1-FLAG R14A (u937) 0 190 10 0 184 16 0 186 14 4C
SAMHD1 (MDM) 196 0 4 192 0 8 198 0 2 AF1A
SAMHD1 (THP1 + PMA) 198 0 2 196 0 4 200 0 0 AF1A
SAMHD1 (Endogenous in Hela) 194 0 6 200 0 0 196 0 4 AF1A
SAMHD1 (Tramsient expression) 198 0 2 200 0 0 194 0 6 AF1B
SAMHD1 (Stable expression 196 0 4 196 0 4 200 0 0 AF1B
SAMHD1-FLAG (15–626) 0 194 6 0 196 4 0 190 10 AF2A
  1. Three independent experiments were performed. Cells were scored visually for nuclear and cytoplasmic distribution. In every experiment two hundred cells were counted. The analysis of the distribution was performed in human HeLa cells or otherwise indicated. MDM = Monocyte derived macrophages. PMA = phorbol-12-myristate-13-acetate.