Role of SAMHD1 nuclear localization in restriction of HIV-1 and SIVmac

Retrovirology

Table 1 Immunostaining data

SAMHD1	Experiment 1			Experiment 2			Experiment 3			Figure
	Exclusively nuclear	Exclusively cytoplasm	Throughout cell	Exclusively nuclear	Exclusively cytoplasm	Throughout cell	Exclusively nuclear	Exclusively cytoplasm	Throughout cell
GFP-SAMHD1	184	0	16	190	0	10	188	0	12	1B
GFP-SAMHD1 (1–328)	176	0	24	180	0	20	177	0	23	1B
GFP-SAMHD1(329-626	0	40	160	0	46	154	0	38	162	1B
GFP-SAMHD1(1–150)	156	0	44	160	0	40	164	0	36	1B
GFP-SAMHD1(151–328)	0	0	200	0	0	200	0	0	200	1B
GFP-SAMHD1 K11A	0	188	12	0	190	10	0	192	8	2B
GFP-SAMHD1 R12A	0	190	10	0	194	6	0	188	12	2B
GFP-SAMHD1 R14A	0	192	8	0	196	4	0	186	14	2B
SAMHD1-FLAG	196	0	4	194	0	6	196	0	4	2D
SAMHD1-FLAG K11A	0	194	6	0	192	8	196	0	4	2D
SAMHD1-FLAG R12A	0	196	4	0	194	6	0	192	4	2D
SAMHD1-FLAG R14A	0	192	8	0	190	10	0	188	12	2D
GFP-muNS	0	200	0	0	200	0	0	200	0	3A
KRPR-GFP-muNS	150	0	50	146	0	54	152	0	48	3A
GFP	0	0	200	0	0	200	0	0	200	3B
KRPR-GFP	140	0	60	146	0	54	142	0	58	3B
SAMHD1-FLAG (U937)	194	0	6	190	0	10	196	0	4	4C
SAMHD1-FLAG K11A (U937)	194	0	6	190	0	10	196	0	4	4C
SAMHD1-FLAG R12A (U937)	0	192	8	0	196	4	0	188	14	4C
SAMHD1-FLAG R14A (u937)	0	190	10	0	184	16	0	186	14	4C
SAMHD1 (MDM)	196	0	4	192	0	8	198	0	2	AF1A
SAMHD1 (THP1 + PMA)	198	0	2	196	0	4	200	0	0	AF1A
SAMHD1 (Endogenous in Hela)	194	0	6	200	0	0	196	0	4	AF1A
SAMHD1 (Tramsient expression)	198	0	2	200	0	0	194	0	6	AF1B
SAMHD1 (Stable expression	196	0	4	196	0	4	200	0	0	AF1B
SAMHD1-FLAG (15–626)	0	194	6	0	196	4	0	190	10	AF2A

Three independent experiments were performed. Cells were scored visually for nuclear and cytoplasmic distribution. In every experiment two hundred cells were counted. The analysis of the distribution was performed in human HeLa cells or otherwise indicated. MDM = Monocyte derived macrophages. PMA = phorbol-12-myristate-13-acetate.

ISSN: 1742-4690