Skip to main content
Figure 7 | Retrovirology

Figure 7

From: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

Figure 7

Neutralisation of FeV by either pooled serum from FeLV-recovered cats or anti-gp70 monoclonal antibody. (A) Purified FeLV-A (Glasgow-1) was separated by SDS-PAGE and transferred to nitrocellulose membrane. Strips of membrane were probed with pooled FeLV-positive sera (lane 1), pooled FeLV-negative sera (lane 2) or murine anti-gp70 MAb (lane 3). 100 I.U. of FeLV-A (Glasgow-1) (A) or the DD, ND and NN mutants were incubated for two hours with serially-diluted pooled (B) FeLV-positive serum or (C) anti-gp70 MAb, before being titrated on QN10 cells. 72 hours post-infection, foci were enumerated manually. Values represent the mean +/− SEM of two independent experiments.

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us