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Figure 5 | Retrovirology

Figure 5

From: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

Figure 5

Expression of soluble Fc-tagged FeLV-SUs. HEK293T cells were transfected with the pTORSTEN vector into which the SU domains of the Glasgow-1 (A), Gardner-Arnstein (B) and Sarma (C), or the Glasgow-1 mutants DD, ND and NN had been cloned. Matched volumes of supernatant were separated by SDS-PAGE, transferred to nitrocellulose and immunoblotted for both gp70 (4-15% gradient gel, upper) and the human IgG Fc tag (8% linear gel, lower).

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Retrovirology

ISSN: 1742-4690

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