Figure 4From: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virusThe DD Env confers enhanced utilisation of THTR1 homologues. MLV (FeLV) lacZ pseudotypes bearing the A (Glasgow-1), C (Sarma), DD, ND or NN Envs were plated onto MDTF cells expressing the feline (fe), human (hu) or porcine (po) THTR1 homologues, human FLVCR1 or human FLVCR2. 72 hours post-infection, cells were stained for expression of lacZ and counted manually. Values represent the mean +/− SEM of three independent experiments. The increase in titre associated with the DD mutation upon feline, human or porcine THTR1 is statistically significant in comparison with A (Glasgow-1) (unpaired T test, p < 0.05).Back to article page