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Figure 3 | Retrovirology

Figure 3

From: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

Figure 3

The DD Env supports more efficient infection of HEK293T cells. (A) MLV(FeLV) lacZ pseudotypes were prepared from HEK293T cells. Samples of each pseudotype preparation were pelleted, separated by SDS-PAGE, immunoblotted and probed with either anti-FeLV gp70 or anti-SFFV Gag. (B) Matched inputs (RT activity) of MLV(FeLV) lacZ pseudotypes bearing the Glasgow-1 (A), DD, ND or NN Envs, were plated onto HEK293T cells. 72 hours post-infection, the cells were stained for expression of lacZ and counted manually. Values are presented as infectious units per μl (I.U./ μl ), mean +/− SE of three independent experiments. The increase in titre noted for the DD mutant and the decrease in titre of the ND mutant (asterisks) are statistically significant in comparison with A (Glasgow-1) (unpaired T test, p < 0.05).

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