Figure 3

Frequency of HTLV-1-infection in each CD4⁺T-cell subset of asymptomatic HTLV-1 carriers. PBMCs from HTLV-1 asymptomatic carriers (n = 23) were cultivated for 18 hours, stained with anti-CD4, anti-CD8, anti-FoxP3, and anti-Tax antibodies, and analyzed by flow cytometry. (A) Representative dot plots of CD4 and CD8 and histograms of Tax in CD4⁺ or CD8⁺ T cells (Left panel). Right, cumulative results from 23 AC individuals are shown in graph (Right panel). (B) Representative histograms of Tax expression in FoxP3⁺ or FoxP3⁻ cell (Left panel). Right, cumulative results from 23 AC individuals are shown in graph (Right panel). (C) Tax positivity in CD4⁺ T cells showed significant correlation with FoxP3 positivity in CD4⁺ T cells by Spearman’s rank correlation (P = 0.0257, r = 0.48)