Nef functions in BLT mice to enhance HIV-1 replication and deplete CD4+CD8+ thymocytes

Retrovirology

Table 1 Relationship between Peak Viral Load, T cell depletion and CD4 ⁺ CD8 ⁺ Thymocyte Depletion ^a

Mouse	Peak Viral Load^b(highest to lowest)	Inoculum (TCIU)^c	T cell Depletion^d	CD4⁺CD8⁺Depletion^e
LAI-7	2.04 × 10⁷	600,000	Yes	Yes
LAI-8	1.19 × 10⁷	600,000	Yes	Yes
LAI-9	1.13 × 10⁷	600,000	Yes	Yes
LAI-4	6.70 × 10⁶	30,000	Yes	Yes
LAI-2	5.63 × 10⁶	3,000	Yes	Yes
LAI-6	4.99 × 10⁶	30,000	Yes	Yes
LAI-3	4.64 × 10⁶	3,000	Yes	Yes
LAI-1	4.25 × 10⁶	3,000	Yes	Yes
LAINefdd-13	4.24 × 10⁶	600,000	Yes	No
LAINefdd-12	3.77 × 10⁶	600,000	Yes	No
LAI-5	3.59 × 10⁶	30,000	Yes	Yes
LAINefdd-14	3.29 × 10⁶	600,000	Yes	No
LAINefdd-11	2.07 × 10⁶	600,000	No	No
LAINefdd-6	1.56 × 10⁶	30,000	No	No
LAINefdd-4	1.25 × 10⁶	3,000	No	No
LAINefdd-2	1.02 × 10⁶	3,000	No	No
LAINefdd-17	9.93 × 10⁵	600,000	No	No
LAINefdd-9	8.82 × 10⁵	600,000	No	No
LAINefdd-16	4.90 × 10⁵	600,000	No	No
LAINefdd-8	4.36 × 10⁵	600,000	No	No
LAINefdd-15	3.00 × 10⁵	600,000	No	No
LAINefdd-1	2.31 × 10⁵	3,000	No	No
LAINefdd-3	2.20 × 10⁵	3,000	No	No
LAINefdd-7	1.91 × 10⁵	30,000	No	No
LAINefdd-10	7.73 × 10⁴	600,000	No	No
LAINefdd-5	6.49 × 10⁴	30,000	No	No

^a Summary of viral loads at peak viremia and depletion of T cell and double positive thymocytes.
^b Copies viral RNA/ml plasma. LAI, 8.15 × 10⁶ ± 1.83 × 10⁶ (n = 9); LAINefdd, 1.24 × 10⁶ ± 0.32 × 10⁶ (n = 17).
^c Dose of intravenous inoculum presented in tissue culture infectious doses (TCIU).
^d “Yes,” mice exhibiting reduction of peripheral blood CD4⁺ T cells below 50% of pre-infection level; viral load, 7.06 × 10⁶ ± 1.47 × 10⁶ (n = 12). “No,” mice exhibiting peripheral blood CD4⁺ T cells not reduced below 50%; viral load, 0.70 × 106 ± 0.16 × 10⁶ (n = 14).
^e “Yes,” CD4⁺CD8⁺ thymocytes reduced below 10% total thymocytes. Viral loads for “Yes” and “No” same as LAI and LAINefdd, respectively (^b).

ISSN: 1742-4690