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Figure 3 | Retrovirology

Figure 3

From: Nef functions in BLT mice to enhance HIV-1 replication and deplete CD4+CD8+ thymocytes

Figure 3

Analysis of BLT humanized mice inoculated with a low dose of nef (−) or wild-type LAI. (A) Each line depicts longitudinal plasma viral load data from individual BLT humanized mice infected with 3000 TCIU of LAINefdd (closed symbols) or LAI (open symbols). These data demonstrate delayed replication of LAINefdd relative to LAI following low dose inoculation. (B) Each line depicts the percentage of CD4+ T cells in peripheral blood over time where each animal’s symbol is matched to the mice in (A). Mice infected with a low dose of LAINefdd showed minimal changes in CD4+ T cell percentages when compared to BLT humanized mice inoculated with an equal dose of LAI. (C) Naïve BLT humanized mice (n = 5 in PB, spleen and HTO or n = 4 in LN, BM, lung and liver) and BLT humanized mice inoculated with 3000 TCIU of LAINefdd (n = 4) exhibited similar levels of CD4+ cells while mice inoculated with the same dose of LAI (n = 3) exhibited a drastic reduction in these cells. Shown are the percentages of human CD4+ T cells present in peripheral blood, lymph nodes, spleen, bone marrow, lung and liver, as well as the percentages of CD4+CD8- and CD4+CD8+ thymocytes in the human thymic organoid. The percent of CD4+ T cells in peripheral blood or tissues was relative to total CD3+ T cells while the percent of CD4+CD8- and CD4+CD8+ thymocytes was relative to total thymocytes. One-way ANOVA with three Bonferroni multiple comparisons tests was performed to compare the results within each tissue. If no difference was detected, the comparison is unmarked (alpha = 0.05). Comparisons yielding significant differences are represented by a line connecting the arrows above the respective bars (**p < 0.01).

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