Figure 1
Increased number of B-cell areas in the intestinal mucosa in acutely SIV-infected macaques. (A) For each of the 13 SIV-infected macaques, viral load was measured in plasma before infection (Pre; filled circles) and every 3 dpi (open circles). Results are expressed as Log10 copies/mL and bars represent median values. (B) CD4+ T-cells were quantified in whole blood collected from each macaque before infection and every 3 dpi. For each animal, CD4+ T-cell counts before infection (filled circles; n=13) and at 11-12 dpi (open circles, n=13) are shown. Results are expressed as cells/μL and bars represent median values. *** p=0.0004 (Wilcoxon test) (C-D) Staining by CD20 mAb of sections from duodenum (C) and terminal ileum (D) of one representative non-infected macaque (Ctl, n=3) and macaques infected for 14 (14 dpi, n=5) or 28 days (28 dpi, n=3). Magnification x25 for all panels (E-F) The number of CD20+ B-cell areas per section was divided by the surface of the total mucosa. Results are expressed as B-cell areas x 10-7/μm2. Each symbol corresponds to one animal. Bars represent median values for each group.