Figure 1
From: The prototype foamy virus protease is active independently of the integrase domain
Proteolytic activity of PFV PR-RT in vitro. (A) The GB1-GFP substrate was incubated at 37°C with PFV PR-RT in the presence or absence of RNAPARM. Reaction products were separated on 10% BisTris gels. M: molecular weight marker, the sizes of the standard proteins are indicated on the left; S: uncleaved substrate. (B) For kinetic studies the conversion of the GB1-GFP substrate was observed by fluorescence anisotropy. The substrate was incubated at 37°C. PFV PR-RT and RNAPARM were added subsequently after 30 and 60 min, respectively.