Figure 6

Sub-cellular localization of HIV-1 antisense RNAs. (A) Strand-specific RT-PCR of fractionated samples. An arrow in the upper panel indicates the region of PCR amplification (R8). Bottom panel, agarose gel electrophoresis of PCR products using samples of HIV-1-infected MAGIC-5A and chronically infected OM10.1 cells. Cyto, cytoplasmic RNA; Nuc, nuclear RNA; sense, results of sense strand-specific RT-PCR at R8. (B–D) Sub-cellular distribution of the asRNAs in various HIV-1-infected cells. Relative levels are presented in percentages for MAGIC-5A, OM10.1, and PHA-blast, as well as simultaneously measured controls. Results of β-actin cytoplasmic RNA and nuclear U3 snoRNA served as controls for fractionation efficiencies.