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Figure 5 | Retrovirology

Figure 5

From: Protein kinase C-delta regulates HIV-1 replication at an early post-entry step in macrophages

Figure 5

Rottlerin reduces cytoskeletal association of HIV-1 RT complexes. (A) Macrophages (5x106) were left untreated or pretreated with rottlerin (Rott) and then infected with HIV-1 BaL (50 ng p24) or VSV-G-pseudotyped vector (10 ng p24). Membrane bound (M) and cytoskeletal (Cs) fractions were obtained and the presence of HIV-1 RT was assessed by Western blotting (upper panels). Purity of membrane bound and cytoskeletal fractions was analyzed by Western blotting for control proteins (bottom panels). (B) Macrophages (5x106) were left untreated or pretreated with rottlerin or cytochalasin D (CCD) and then infected with HIV-1 BaL (50 ng p24). Membrane bound (M), cytoskeletal (Cs) and nuclear (N) fractions were obtained, and the presence of Gag-MA was assessed by Western blotting (upper panels). The purity of membrane bound, cytoskeletal and nuclear fractions was analyzed by Western blotting for control proteins (bottom panels).

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