Figure 4

Actin cytoskeleton is altered by pretreatment of macrophages by rottlerin (5 μM). Human macrophages were treated for 24 h with rottlerin (5 μM, C), transfected with siRNAs to PKC-delta (D) or left untreated (A and B) and then fixed with formaldehyde (3.7%). Macrophages were then permeabilised with Triton X-100 (0.2%) and stained with rhodamine-phalloidin before mounting using moviol. Preparations were then observed by confocal microscopy. Confocal images are representative of three independent experiments. Arrows indicate stress fibers (A) and pseudopods (B). (E) Quantitative results represent the percentage of cells with proper actin cytoskeleton in the presence of different inhibitors or after treatment with siRNA against PKC-delta.