Figure 4

Sequence specific requirement of V-G-F for Nef function. Panels A to C show relative surface levels of CD4, MHC-I, CXCR4 respectively, measured by flow cytometry on Jurkat CD4-CCR5 cells transduced with retroviral vectors expressing either eGFP alone or together with the indicated nef alleles and mutants. To determine relative surface levels, the MFI of transduced cells (eGFP⁺) was divided by the MFI of untransduced cells (eGFP^-) in the same culture. Data represent the mean relative expression and standard deviations obtained from 3 independent experiments,** over a bar indicates P < 0.01 and *** indicates P < 0.001.