Table 1 Cellular proteins detected exclusively in wild-type Nef immunocomplexes

FACS-sorted 5C Nef

Q9NV70

EXOC1

EXOC1

Exocyst complex component 1, isoform 1

3

Q96KP1

EXOC2

EXOC2

Exocyst complex component 2

3

O60645

EXOC3

EXOC3

Exocyst complex component 3, isoform 1

3

Q96A65

EXOC4

EXOC4

Exocyst complex component 4

6

Q8TAG9

EXOC6

EXOC6

Exocyst complex component 6

1

Q9UL85 ^a

Myosin-reactive Igκ chain variable region (fragment)

1

P19838

NFKB1

NFKB1

Nuclear factor NF-κB p105 subunit, isoform 2

1

Unsorted 5C Nef

Q9NV70

EXOC1

EXOC1

Exocyst complex component 1, isoform 1

3

Q96KP1

EXOC2

EXOC2

Exocyst complex component 2

3

O60645

EXOC3

EXOC3

Exocyst complex component 3, isoform 1

2

Q96A65

EXOC4

EXOC4

Exocyst complex component 4

4

Q9NP29 ^a

Microfibrillar protein 2 (fragment)

1

Q13177

PAK2

Pak2

PAK2 Serine/threonine-protein kinase

3

P60866

RPS20

RS20

40S ribosomal protein S20

1

Gene or Accession

D ^N -score ^b

Z-score ^b

Description

Total #peptides

Unique # peptides

FACS-sorted and unsorted 5C Nef combined

EXOC1

7.14

10.09

Exocyst complex component 1, isoform 1

6

5

EXOC2

7.14

10.09

Exocyst complex component 2

6

5

EXOC3

5.83

10.09

Exocyst complex component 3, isoform 1

4

4

EXOC4

9.21

10.10

Exocyst complex component 4

10

6

EXOC6

0.41

10.05

Exocyst complex component 6

1

1

NFKB1

0.05

−0.59

Nuclear factor NF-κB p105 subunit, isoform 2

1

1

PAK2

0.70

10.09

PAK2 Serine/threonine-protein kinase

3

3

Q9NP29 ^a

0.41

10.05

Microfibrillar protein 2 (fragment)

1

1

Q9UL85 ^a

0.41

10.05

Myosin-reactive Igκ chain variable region (fragment)

1

1

RPS20

0.04

−1.51

40S ribosomal protein S20

1

1