Synthesis and integration of SIV DNA in primary human macrophages. Wild type and Vpx mutant SIVs were pseudotyped with VSV-G and used to infect M-CSF-stimulated human monocyte-derived macrophages. After 24 or 48 hrs, total cellular DNA was harvested and subjected to PCR reactions for A) early or B) late reverse transcription products, or C) levels of integrated viral DNA, performed in triplicate assays. Levels of viral DNAs are relative to the average obtained with wild type SIVmne at each time point. The bars in each set of assays indicate standard deviations of assays.