Figure 3

Effect of SPG21 and FANCG knockdown on viral promoter activation. Jurkat-LTR-Luc cells were transduced with lentiviral particles expressing a control shRNA and three validated shRNAs targeting various sequences of the SPG21 and FANCG mRNAs. Cells were cultured for 24 hours, and luciferase activities were determined from cell lysates and normalized to corresponding cell viability data (measured by WST1 test). Results are means of three experiments and error bars indicate standard errors.