Table 2 Parameters values and derived quantities for the in vitro experiment
Parameter Name | Symbol | Unit | Value | 95%CI |
|---|---|---|---|---|
Calculated parameters for the continuous approximation of cell and virus harvest | ||||
Harvest rate of target and infected cells | δ | day-1 | 0.057 | -- |
Harvest rate of total and infectious virus | r c | day-1 | 7.31 | -- |
Fitted parameters from separate experiments | ||||
Decay rate of uninfected cells | d | day-1 | 0.21 | 0.17-0.26 |
Rate of virion infectivity loss | r I | day-1 | 0.93 | 0.44-1.4 |
Degradation rate of virion RNA | r RNA | day-1 | 0.039 | 0.013-0.065 |
Parameters obtained from simultaneous fit to full in vitro dataset | ||||
Rate constant for infections | β 50 | (TCID50/ml·day)-1 | 4.95 × 10-5 | (2.35-9.59) × 10-5 |
Decay rate of infected cells | a | day-1 | 1.18 | 0.85-1.26 |
Production rate of total virus | k | RNA copies·day-1 | 2.61 × 104 | (1.55-3.70) × 104 |
Production rate of infectious virus | k 50 | TCID50·day-1 | 0.22 | 0.12-0.40 |
Quantities derived from fitted values | ||||
Viral burst size (total) | k/a | RNA copies | 2.21 × 104 | (1.74-2.96) × 104 |
Viral burst size (infectious) | k 50 /a | TCID50 | 0.19 | 0.11-0.33 |
Basic reproductive number (without removal) | R 0 | -- | 62.8 | 51.1-76.8 |
Basic reproductive number (with removal) | R 0 * | -- | 7.01 | 5.70-8.45 |
Minimum fraction of infectious virus | k 50 /k | TCID50/RNA copies | 8.63 × 10-6 | (4.53-16.9) × 10-6 |