Characterization of AnkGAG1D4. (A), Amino acid sequence of the AnkGAG1D4 protein, represented by using the single letter code. The variable residues at the six predefined positions on the ankyrin solvent-exposed surface were highlighted in red. (B), SDS-PAGE analysis of AnkGAG1D4. The AnkGAG1D4 protein was first isolated by affinity chromatography on nickel-column (HisTrap column; lane 3), and further purified by gel filtration chromatography (Sephadex G-75 column; lane 2). Lane 1, molecular mass markers.