Figure 3
From: Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR
Spt6, PAAF1 and RNAPII localize at sites of HIV-1 transcription. (A) U2OS-LTR-MS2 cells transfected with pMS2-cherry and pTat-Flag were fixed, and analyzed by immunofluorescence using the indicated antibodies. Sites of co-localization with MS2-labelled HIV-1 transcription sites are indicated by arrowheads. (B) Anti-PAAF1 antibody specifically recognizes endogenous PAAF1. U2OS-LTR-MS2 cells were transfected with control (scr) or PAAF1-specific siRNA, and fixed 48 hr later. Cells were pre-treated with 0.05% Triton/PBS, fixed and stained with anti-PAAF1 antibody (red) for immunofluorescence analysis.