Figure 2
From: Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR
PAAF1 interacts with the N-terminus of Spt6. (A) Extracts from 293T transfected with PAAF1-Flag alone or together with Myc-Spt6 were analyzed by IP-IB using the indicated antibodies. (B) 293T extract was immunoprecipitated using anti-Spt6 followed by IB using anti-PAAF1. (C) Extracts from 293T transfected with PAAF1-Flag and the indicated Spt6-Myc constructs were immunoprecipitated using anti-Myc or IgG followed by IB with anti-Flag.