Figure 1
From: Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR
Spt6 levels are controlled by PAAF1 in a proteasome-dependent manner. (A) HeLa cells transfected with siRNA against PAAF1 or a control (Scr) were analyzed by IB using the indicated antibodies. (B) Extracts from HeLa transfected with the indicated siRNAs and treated with MG132 where indicated were analyzed by IB using the indicated antibodies. (C) Spt6 association with HIV-1 chromatin is diminished by ablation of PAAF1. HeLa-LTR-luc transfected with the indicated siRNAs were analyzed by IB using the indicated antibodies, and ChIP using anti-Spt6. Sequences within the HIV promoter and luciferase region were amplified by Q-PCR. The value for the Scr sample in the promoter region was set to 1. Locations of primers used for PCR amplification are indicated above the graph. (D) Cells in B were analyzed by ChIP as described in C using anti-Spt6. Graphs show mean +/- SD (n = 3).