Table 1 Primers used for PCR amplification analysis

feline _{GAPDH for}

5' AAA TTC CAC GGC ACA GTC AAG

NA

Feline GAPDH QT PCR

feline _{GAPDH rev}

5' TGA TGG GCT TTC CAT TGA TGA

NA

Feline GAPDH QT PCR

18s _{rRNA for}

5' GTA ACC CGT TGA ACC CCA TT

NA

Feline 18s rRNA

18s _{rRNA rev}

5' CCA TCC AAT CGG TAG TAG CG

NA

Feline 18s rRNA

FIV _{QT gag for}

5' TAG CCC TTG ACC CAA AAA TG

1067

FIVgag QT PCR

FIV _{QT gag rev}

5' ATT GGC CGA AAA AGC TGT AA

1167

FIVgag QT PCR

FIV _{Gag leader for}

5' GTT GGC GCCCGA ACA GGG

354

FIV gag sequencing

Gag _{circle rev}

5' GTA GAT GGT CTG GTG TCT AAT CCC A

989

FIV gag sequencing

FIV _{U5 rev}

5' TGC GAA GTC TTC GGC CCG GAC TCC G

338

FIV LTR sequencing

FIV _{env for}

5' TGG GAG TCC TCT GAC CGA GA

9043

FIV LTR sequencing + CJ A and B

FIV _{gag circle rev3}

5' GGT TTC ACT TTC CTA GCC TGC TC

403

CJ B

FIV _{gag rev}

5' TGA GTC AGC CCT ATC CCC ATT A

1345

CJ A

FIV _{u5 for}

5' CCT GTC GTG TAT CTG TGT AAT CTT TTC TAA C

290

CJ C

FIV _{u3 rev}

5' TGG AAC AAA ATC TAC GTC ATC GG

155

CJ C