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Table 1 Primers used for PCR amplification analysis

From: FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes in vivo during the asymptomatic phase of infection

Primer name Sequence FIV genomic position * Assay
feline GAPDH for 5' AAA TTC CAC GGC ACA GTC AAG NA Feline GAPDH QT PCR
feline GAPDH rev 5' TGA TGG GCT TTC CAT TGA TGA NA Feline GAPDH QT PCR
18s rRNA for 5' GTA ACC CGT TGA ACC CCA TT NA Feline 18s rRNA
18s rRNA rev 5' CCA TCC AAT CGG TAG TAG CG NA Feline 18s rRNA
FIV QT gag for 5' TAG CCC TTG ACC CAA AAA TG 1067 FIVgag QT PCR
FIV QT gag rev 5' ATT GGC CGA AAA AGC TGT AA 1167 FIVgag QT PCR
FIV Gag leader for 5' GTT GGC GCCCGA ACA GGG 354 FIV gag sequencing
Gag circle rev 5' GTA GAT GGT CTG GTG TCT AAT CCC A 989 FIV gag sequencing
FIV U5 rev 5' TGC GAA GTC TTC GGC CCG GAC TCC G 338 FIV LTR sequencing
FIV env for 5' TGG GAG TCC TCT GAC CGA GA 9043 FIV LTR sequencing + CJ A and B
FIV gag circle rev3 5' GGT TTC ACT TTC CTA GCC TGC TC 403 CJ B
FIV gag rev 5' TGA GTC AGC CCT ATC CCC ATT A 1345 CJ A
FIV u5 for 5' CCT GTC GTG TAT CTG TGT AAT CTT TTC TAA C 290 CJ C
FIV u3 rev 5' TGG AAC AAA ATC TAC GTC ATC GG 155 CJ C
  1. QT PCR = real time PCR; CJ = circle junctions PCR; NA = not applicable
  2. * 5' position of FIV primer