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Figure 5 | Retrovirology

Figure 5

From: FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes in vivo during the asymptomatic phase of infection

Figure 5

Cloned viral 2-LTR circle junction sequences. PBMCs were isolated from FIV-infected cats 6 weeks (184) and 12 weeks (165) PI and cultured ex vivo. DNA was isolated from ex vivo-cultured cells and 2-LTR CJ PCR was performed (CJ primer set C). 2-LTR CJ PCR products were cloned and sequenced from cat 184 PBMCs incubated ex vivo for 3 days (*) and from cat 165 PBMCs incubated 4 (#) and 6 days (+). Circled nucleotides represent the invariant CA and TG dinucleotides within the U5 and U3 regions, respectively, while the underlined nucleotides represent a portion of the primer binding site within the gag leader.

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