Kinetics analysis of SAMHD1 protein and mRNA in DCs treated with IFNα. Monocyte-derived DCs (MDDC) from two independent donors were mock treated, indicated with “-”, or treated with 2000 U/mL IFNα, indicated with “+”, for the indicated times. (A) Whole cell lysates were subjected to immunoblotting for SAMHD1 and tetherin expression. GAPDH was used as a loading control. (B) Quantitative PCR was performed using SAMHD1 cDNA specific primers and all data was normalized to GAPDH. Data shown represents fold change in mRNA levels compared to 0 hr mock treated cells.