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Figure 5 | Retrovirology

Figure 5

From: SAMHD1 restricts HIV-1 infection in dendritic cells (DCs) by dNTP depletion, but its expression in DCs and primary CD4+T-lymphocytes cannot be upregulated by interferons

Figure 5

Kinetics analysis of SAMHD1 protein and mRNA in DCs treated with IFNα. Monocyte-derived DCs (MDDC) from two independent donors were mock treated, indicated with “-”, or treated with 2000 U/mL IFNα, indicated with “+”, for the indicated times. (A) Whole cell lysates were subjected to immunoblotting for SAMHD1 and tetherin expression. GAPDH was used as a loading control. (B) Quantitative PCR was performed using SAMHD1 cDNA specific primers and all data was normalized to GAPDH. Data shown represents fold change in mRNA levels compared to 0 hr mock treated cells.

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Retrovirology

ISSN: 1742-4690

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