Type I and type II IFN treatment upregulates SAMHD1 mRNA levels in DCs at 6 hr post-treatment. Monocyte-derived DCs (MDDC) from two independent donors were either mock treated, or treated with 2000 U/mL of (A) IFNα, (B) IFNβ or (C) IFNγ as indicated for either 6 or 24 hr. Cell pellets were collected and total RNA was extracted and 200 ng of the total RNA were used for cDNA synthesis. Quantitative PCR was performed using SAMHD1 cDNA specific primers and all data was normalized to GAPDH. Data shown represent fold change in mRNA levels compared to 6 hr mock treated cells. The asterisks indicate a significant difference (p <0.05) compared with the mock controls.