Type I and type II IFN treatment does not upregulate SAMHD1 expression in DCs. (A and B) Monocyte-derived DCs (MDDCs) were treated with different amounts of IFNα or IFNβ as indicated for 24 hr and whole cell lysates were subjected to immunoblotting for SAMHD1 and tetherin expression. GAPDH or β-actin was used as a loading control. (C) MDDCs were mock treated or treated with IFNγ at the indicated concentrations for 24 hours. Whole cell lysates of treated MDDCs were subjected to immunoblotting for SAMHD1 or HLA-II. β-actin was used as a loading control. The MDDC data shown represents one of two independent experiments using cells from different donors.