Inhibition of gp41 6-HB formation by 2DLT. A) Binding of D1D2, 2DLT, and T1144 to the grooves on the 5-helix as assessed by ELISA. B) The inhibition of D1D2, 2DLT, and T1144 to the 6-HB formation between N36 and C34-biotin was detected by ELISA. Each sample was tested in triplicate, and the data are presented as means ± SD. C) The inhibition of D1D2 and 2DLT at graded concentration to the 6-HB formation between N36 and C34-FAM was determined by FN-PAGE. The peptide N36 was incubated with 2DLT or D1D2 at 37°C for 30 minutes before addition of the peptide C34-FAM at graded concentrations. After incubation for another 30 minutes, the mixtures were analyzed by FN-PAGE (panels a and b). The gels in panels a and b were stained with Coomassie Blue (panels c and d).